Laboratory of Reproductive Physiology

Tetraspanins are proteins of cell membranes defined by a specific structure. Their characteristic feature is the ability to associate with other tetraspanins, proteins and also lipids to form functional tetraspanin web affecting cell morphology, as well as fusion, motility, signalling and other cellular processes. It has been shown that tetraspanins are also involved in the fertilization process in mammals; they act within the complexes necessary for successful fertilization and zygote formation. The Laboratory of Reproductive Physiology is primarily concerned with the study of the structure and dynamics of tetraspanin web of bovine gametes (oocytes and sperm) and developmental stages during their maturation in the genital organs, ovaries, testes and epididymides. We also study the localization and distribution of tetraspanins during the processes determining the ability of ejaculated sperm to pass through the zona pellucida and penetrate the oocyte cytoplasm by stimulation of sperm capacitation and acrosome reaction in vitro and moreover, during early embryonic development using fertilization in vitro.
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RNDr. Jana Antalíková, PhD.
Ing. Jana Jankovičová, PhD.
Ing. Katarína Michalková, PhD.
Ing. Petra Sečová, PhD.
Ing. Ľubica Horovská
Bc. Claudia Feitscherová – diplomantka
Jana Komárová – bakalárka
Vladimíra Kabaňová – bakalárka


Laboratory of Reproductive Biology, Institute of Biotechnology CAS, v.v.i., BIOCEV, 252 50 Vestec, Czech Republic

Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 165 00 Prague 6, Czech Republic

National Agricultural and Food Centre Research Institute for Animal Production Nitra, Hlohovecká 2, 951 41 Lužianky, Slovak Republic

Selected publications

JANKOVIČOVÁ J.; SEČOVÁ P.; MICHALKOVÁ K.; ANTALÍKOVÁ J.: Tetraspanins, More than Markers of Extracellular Vesicles in Reproduction. International Journal of Molecular Science. 2020, 21, 7568.

JANKOVIČOVÁ J., NEUEROVÁ Z., SEČOVÁ P., BARTÓKOVÁ M., BUBENÍČKOVÁ F., KOMRSKOVÁ K., POSTLEROVÁ P., ANTALÍKOVÁ J.: Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos. Medical Microbiology and Immunology. 2020, 209:407–425,

JANKOVICOVA J., FROLIKOVA M., PALENIKOVA V., VALASKOVA E., CERNY J., SECOVA P., BARTOKOVA M., HOROVSKA L., MANASKOVA-POSTLEROVA P., KOMRSKOVA K., ANTALIKOVA J.: Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells. Scientific Reports. 2020, 10:4374 (4.011- IF2018)

ANTALIKOVA J., SECOVA P., HOROVSKA L., KREJCIROVA R., SIMONIK O., JANKOVICOVA J., BARTOKOVA M., TUMOVA L., MANASKOVA-POSTLEROVA P.: Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa? Cells. 2020, 9, 183; (5.656-IF 2018)

JANKOVIČOVÁ J., SEČOVÁ P., MAŇÁSKOVÁ-POSTLEROVÁ P., ŠIMONÍK O., FROLÍKOVÁ M., CHMELÍKOVÁ E., HOROVSKÁ Ľ., MICHALKOVÁ K., DVOŘÁKOVÁ-HORTOVÁ K., ANTALÍKOVÁ J.: Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos. International Journal of Biological Macromolecules, 2019, vol. 123, p. 931-938. (4.784-IF2018),

FROLÍKOVÁ M., MAŇÁSKOVÁ-POSTLEROVÁ P., ČERNÝ J., JANKOVIČOVÁ J., ŠIMONÍK O., POHLOVÁ A., SEČOVÁ P., ANTALÍKOVÁ J., DVOŘÁKOVÁ-HORTOVÁ K.: CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization. International Journal of Molecular Sciences, 2018, vol. 19, iss. 4, art. no. 1236. (3.687 – IF2017),

JANKOVIČOVÁ J., MICHALKOVÁ K., SEČOVÁ P., HOROVSKÁ Ľ., MAŇÁSKOVÁ-POSTLEROVÁ P., ANTALÍKOVÁ J.: Evaluation of protein phosphorylation in bull sperm during their maturation in the epididymis. Cell and Tissue Research, 2018, vol. 371, no. 2, p. 365-373. (3.043 – IF 2017),

JANKOVIČOVÁ J., FROLIKOVÁ M., ŠEBKOVÁ N., SIMON M., CUPPEROVÁ P., LIPCSEYOVÁ D., MICHALKOVÁ K., HOROVSKÁ Ľ., SEDLÁČEK R., STOPKA P., ANTALÍKOVÁ J., DVOŘÁKOVÁ-HORTOVÁ K.: Characterization of tetraspanin protein CD81 in mouse spermatozoa and bovine gametes. Reproduction, 2016, vol. 152, no. 6, p. 785-793. (3.184 – IF2015). (2016 – Current Contents). ISSN 1470-1626,

ANTALÍKOVÁ J., JANKOVIČOVÁ J, SIMON M., CUPPEROVÁ P., MICHALKOVÁ K., HOROVSKÁ Ľ.: Localization of CD9 molecule on bull spermatozoa; itsinvolvement in the sperm – egg interaction. Reproduction in domestic animals. 2015, 50, 423–430; (1,177-IF2013) ISSN 0936–6768,

JANKOVIČOVÁ J., SIMON M., ANTALÍKOVÁ J., CUPPEROVÁ P., MICHALKOVÁ K.: Role of tetraspanin CD9 molecule in fertilization of mammals. Physiological Research. 2015, 64: 279-293, (1,487-IF2013), doi: 10.33549/physiolres.932876.

CUPPEROVÁ P., SIMON M., ANTALÍKOVÁ J., MICHALKOVÁ K., HOROVSKÁ Ľ., HLUCHÝ S.: Distribution of tetraspanin family protein CD9 in bull reproductive system
Czech Journal of Animal Science, 59, 2014 (3): 134–139 (0.871 – IF2013). (2014 – Current Contents). ISSN 1212-1819,

MICHALKOVÁ K., SIMON M., ANTALÍKOVÁ J., KLÍMA J., HOROVSKÁ Ľ., JANKOVIČOVÁ J., HLUCHÝ S.: Identification of bovine CD52-like molecule by monoclonal antibody IVA-543 : distribution of CD52- like molecule in the bull genital tract. Theriogenology. 2010, vol. 74, no. 6, p. 1066-1074. (2.073 – IF2009). (2010 – Current Contents). ISSN 0093-691X,